proteintech rabbit polyclonal txnl6  (Proteintech)

Journal: PLoS ONE

Article Title: TXNL6 Is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of α-Crystallin and Cytochrome C in the Eye Lens

doi: 10.1371/journal.pone.0015421

Figure Lengend Snippet: Ethidium bromide stained agarose gels show Panel A - TXNL6, Panel B - Trx 1, Panel C - Trx 2 and Panel D GAPDH (control) transcript from 200 ng of RNA obtained from human tissues - 1. Lens epithelium, 2. Lens Fiber, 3. Retina, 4. Stomach, 5. Kidney, 6.Heart, 7. Colon, and 8. Spleen.

Article Snippet: The resulting TXNL6 protein was examined by SDS-PAGE and western blot analysis using the ProteinTech rabbit polyclonal TXNL6-specific antibody for purity and identity.

Techniques: Staining

Journal: PLoS ONE

Article Title: TXNL6 Is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of α-Crystallin and Cytochrome C in the Eye Lens

doi: 10.1371/journal.pone.0015421

Figure Lengend Snippet: SDS-PAGE and immunoblotting of microdissected lens epithelium (Epi) and lens fiber cell total protein extracts (20 µg) with a TXNL6-specific antibody.

Article Snippet: The resulting TXNL6 protein was examined by SDS-PAGE and western blot analysis using the ProteinTech rabbit polyclonal TXNL6-specific antibody for purity and identity.

Techniques: SDS Page, Western Blot

Journal: PLoS ONE

Article Title: TXNL6 Is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of α-Crystallin and Cytochrome C in the Eye Lens

doi: 10.1371/journal.pone.0015421

Figure Lengend Snippet: SDS-PAGE and immunoblotting of Panel A - TXNL6, Panel B - MsrA, Panel C - Trx 1, and Panel D -Trx 2 in 5 µg of protein extracts from the cytoplasm and mitochondria of HLE-B3 lens epithelial cells using specific antibodies.

Article Snippet: The resulting TXNL6 protein was examined by SDS-PAGE and western blot analysis using the ProteinTech rabbit polyclonal TXNL6-specific antibody for purity and identity.

Techniques: SDS Page, Western Blot

Journal: PLoS ONE

Article Title: TXNL6 Is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of α-Crystallin and Cytochrome C in the Eye Lens

doi: 10.1371/journal.pone.0015421

Figure Lengend Snippet: Co-localization of Panel A - TXNL6 (green), Panel B - Trx 1 (green) and Panel C - Trx 2 (green), mitotracker red – a specific mitochondrial marker and merging of the two images (orange/yellow) in HLE-B3 lens epithelial cells by immunofluoresence microscopy.

Article Snippet: The resulting TXNL6 protein was examined by SDS-PAGE and western blot analysis using the ProteinTech rabbit polyclonal TXNL6-specific antibody for purity and identity.

Techniques: Marker, Microscopy

Journal: PLoS ONE

Article Title: TXNL6 Is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of α-Crystallin and Cytochrome C in the Eye Lens

doi: 10.1371/journal.pone.0015421

Figure Lengend Snippet: A. Ethidium bromide stained agarose gels showing TXNL6 and control GAPDH transcript levels in 200 ng RNA isolated from HLE-B3 human lens epithelial cells at 0, 30 min, 6 h and 16 h recovery following a 2 h exposure to 200 µM H 2 O 2 . B. Western blot showing TXNL6 protein levels in 5 µg of total protein extract isolated from HLE-B3 lens epithelial cells at 0, 6 h, 16 h and 24 h recovery following a 2 h exposure to 200 µM H 2 O 2 . The coomassie blue stained SDS-PAGE gel is shown as a control for equal protein loading.

Article Snippet: The resulting TXNL6 protein was examined by SDS-PAGE and western blot analysis using the ProteinTech rabbit polyclonal TXNL6-specific antibody for purity and identity.

Techniques: Staining, Isolation, Western Blot, SDS Page

Journal: PLoS ONE

Article Title: TXNL6 Is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of α-Crystallin and Cytochrome C in the Eye Lens

doi: 10.1371/journal.pone.0015421

Figure Lengend Snippet: Representative graph (from 3 independent experiments) of cytochrome c (cyt c) mediated peroxidase activity. The graph shows mean values for an N of 3 ± SD. Cyt c oxidized (cyt c ox) (incubated for 15 min with a 4∶1 molar ratio of HOCl) is 100% peroxidase activity, all other activities are expressed as a percentage of the cyt c ox activity. The untreated cyt c protein has 16% cyt c peroxidase activity compared to cyt c ox. Cyt c ox incubated with MsrA in the absence of a reducing system has 91% activity of the oxidized. Treatment of the cyt c ox (291 µM) with MsrA (1.9 µM for 2 h at 37°C) and TXNL6 (1.39 µM) with thioredoxin reductase (TxrR) and NADPH leads to a statistically significant (p<0.001) 48% decrease in peroxidase activity. Similarly treatment of cyt c ox with MsrA and either Trx 1 or Trx 2 (both 1.39 µM) with TxrR/NADPH also significantly (p<0.001) decreased both peroxidase activities by 52% and 54% respectively. Incubation of cyt c ox with MsrA and TXNL6 in the absence of TxrR/NADPH resulted in just a 14% decrease in cyt c peroxidase activity while incubation of cyt c ox with TXNL6 and TxrR/NADPH in the absence of MsrA resulted in 19% decrease in cyt c peroxidase activity. p values were obtained using Tukey's test following one-way ANOVA.

Article Snippet: The resulting TXNL6 protein was examined by SDS-PAGE and western blot analysis using the ProteinTech rabbit polyclonal TXNL6-specific antibody for purity and identity.

Techniques: Activity Assay, Incubation

Journal: PLoS ONE

Article Title: TXNL6 Is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of α-Crystallin and Cytochrome C in the Eye Lens

doi: 10.1371/journal.pone.0015421

Figure Lengend Snippet: Coomassie staining of a Tricine-SDS-PAGE gel following CNBr cleavage of oxidized cyt c (5 µg). Lane 1 untreated cyt c cleaved with CNBr. Lane 2 Oxidized cyt c (0.2 mM oxidized with 0.8 mM HOCl; a 4∶1 Molar ratio) cleaved with CNBr. Lane 3 Oxidized cyt c (291 µM) treated with MsrA (1.9 µM) and DTT (15 mM) for 2 h at 37°C and cleaved with CNBr. Lane 4 Oxidized cyt c (291 µM) treated with MsrA (1.9 µM) and TXNL6 (10 µg; 1.39 µM) for 2 h at 37°C and cleaved with CNBr. RA - reducing agent/system.

Article Snippet: The resulting TXNL6 protein was examined by SDS-PAGE and western blot analysis using the ProteinTech rabbit polyclonal TXNL6-specific antibody for purity and identity.

Techniques: Staining, SDS Page

Journal: PLoS ONE

Article Title: TXNL6 Is a Novel Oxidative Stress-Induced Reducing System for Methionine Sulfoxide Reductase A Repair of α-Crystallin and Cytochrome C in the Eye Lens

doi: 10.1371/journal.pone.0015421

Figure Lengend Snippet: Coomassie staining of a Tricine-SDS-PAGE gel following CNBr cleavage of oxidized α-crystallin (5 µg). Lane 1 untreated α-crystallin cleaved with CNBr. Lane 2 Oxidized α-crystallin (9.09 µM oxidized with 909 µm HOCl; a 100∶1 Molar ratio) cleaved with CNBr. Lane 3 Oxidized α-crystallin (6.4 µM) treated with MsrA (100 nM) and DTT (15 mM) for 2 h at 37°C and cleaved with CNBr. Lane 4 Oxidized α-crystallin (6.4 µM) treated with MsrA (100 nM) and TXNL6 (10 µg; 1.39 µM) for 2 h at 37°C and cleaved with CNBr. Lane 5 Oxidized α-crystallin (6.4 µM) treated with MsrA (100 nM) and TXNL6 (20 µg; 2.78 µM) for 2 h at 37°C and cleaved with CNBr. RA - reducing agent/system.

Article Snippet: The resulting TXNL6 protein was examined by SDS-PAGE and western blot analysis using the ProteinTech rabbit polyclonal TXNL6-specific antibody for purity and identity.

Techniques: Staining, SDS Page